PubReading [333] - Enzymatic assembly of DNA molecules up to several hundred kilobases - D. Gibson, H. Smith et al.

PubReading
31/05/2023 10 min Temporada 1 Episodio 300
PubReading [333] - Enzymatic assembly of DNA molecules up to several hundred kilobases - D. Gibson, H. Smith et al.

Listen "PubReading [333] - Enzymatic assembly of DNA molecules up to several hundred kilobases - D. Gibson, H. Smith et al."

Descargar episodio Ver en sitio original

Episode Synopsis

We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5' exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool.DOI:10.1038/NMETH.1318 - 2009

More episodes of the podcast PubReading

PubReading [327] - DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access - B. Bögels, T. de Greef et al. 17/05/2023
PubReading [346] - Transparency Is the Key to Quality - A. Fosang & R. Colbran 03/07/2023
PubReading [343] - The changing career trajectories of new parents in STEM - E. Cech & M. Blair-Loy 26/06/2023
PubReading [340] - The application of human tissue for drug discovery and development - M. Clotworthy 19/06/2023
PubReading [334] - Open science challenges, benefits and tips in early career and beyond - C. Allen & D. Mehler 05/06/2023
PubReading [331] - Addressing Isolation in the Scientific Community - D. Tomasello 29/05/2023
PubReading [328] - Reflections on scientific collaboration between basic researchers and clinicians - J. Muia & C. Casari 22/05/2023
PubReading [325] - The Costs of Reproducibility - R. Poldrack 15/05/2023
PubReading [323] - Factors that influence PhD candidates’ success: the importance of PhD project characteristics - E. van Rooij, M. Fokkens-Bruinsma & E. Jansen 08/05/2023
PubReading [320] - Deep Neural Networks as Scientific Models - R. Cichy and D. Kaiser 01/05/2023
© 2003-2025 ZARZA | AHEAD OF OUR TIME
privacy policy
In God we trust In God we trust
PreSales Questions? Click Here
or call our toll-free line at +1-888-5002068 Miami, Florida, USA