Listen "Revitalizing the western blot"
Episode Synopsis
Love it or hate it, western blotting forms the bedrock of countless studies across numerous disciplines. Explore its history, development and applications in this episode all about the marmite of life science techniques.Guiding me through the hand wringing, hair follicle destroying history and process of western blotting, and hopefully explaining the beauty and potential of the technique - is Kenneth Oh, Senior Project Manager at Bio-Rad Laboratories. Kenneth reveals some of the latest developments in western blotting. Revealing how researchers are now able to validate the success of each stage of a western blot and work with smaller samples, Kenneth provides key tips for ensuring each of your western blots is a triumph. Contents:Intro: 00:00-01:15History of western blotting: 01:15-3:50What makes western blotting so tricky? 03:50-05:30Stepwise optimization: 05:30-06:50Stain-free western blotting: 06:50-08:50The different variants of western blotting: 08:50-10:20The right blot for the right application: 10:20-11:30The most exciting developments in western bloting:11:30-13:35New horizons for western blotting: 13:35-15:25The impact of multiplex-western blotting: 15:25-16:30Tips for best practice: 16:30-18:10The future of western blotting: 18:10-20:30Yearning for automation: 20:30-21:30 Hosted on Acast. See acast.com/privacy for more information.
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