E2: Pyrosequencing

14/07/2023 34 min

Listen "E2: Pyrosequencing"

Episode Synopsis

Daniel and Iuliia are confused about fireflies- Scientific american on how fireflies create light:https://www.scientificamerican.com/article/how-and-why-do-fireflies/- A small breakdown of a typical Pyrosequencing workflow:https://www.news-medical.net/life-sciences/What-is-Pyrosequencing.aspx- Biology-pages on Pyrosequencing: https://www.biology-pages.info/P/Pyrosequencing.html- Biotechfront on Pyrosequencing:https://www.biotechfront.com/2021/04/pyrosequencing-principle-and-steps.html- Shomu's Biology on Pyrosequencing: https://www.youtube.com/watch?v=he6kJStE2zs- Quick Biochemistry Basics explaining the method:https://www.youtube.com/watch?v=wY8to-_zAEo- Bionity on Pyrosequencing:https://www.bionity.com/en/encyclopedia/454_Life_Sciences.htmlChapters:00:00 Intro03:00 Fireflies04:00 Energy06:00 Luciferase Reaction11:00 TTTTTTTTTTTTTTT...T?14:00 microwell Plates18:00 Pyrosequencing25:00 Storage & Price30:00 Teaser31:00 Pyro?34:00 OutroSince 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation SequencingCompetence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany.Listen to our podcast and we're happy to receive yourfeedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).The Explain Podcast is recorded at the Medienlabor at the Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomicsfacility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).License NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠Creative Commons Attribution 4.0 International License⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: ⁠⁠https://ngs-cn.de/explain-podcast/