Supersizing Molecular Cloning by Rewriting the Rules for Golden Gate Assembly

NEBinspired blog
15/08/2023 13 min
Supersizing Molecular Cloning by Rewriting the Rules for Golden Gate Assembly

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Episode Synopsis

Hear about how NEB scientists have rewritten the rules for Golden Gate Assembly of large multi-fragment targets - using Data-optimized Assembly Design (DAD) and modified protocols they have assembled the entire T7 bacteriophage genome from 52 fragments.

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