Separation and Purification Techniques

26/11/2025 1h 3min

Listen "Separation and Purification Techniques"

Episode Synopsis

This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells.  Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC). For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics.    Visit MedSchoolCoach.com for more help with the MCAT.    Jump into the conversation: [00:00] MCAT Tutorig from MedSchoolCoach [00:34] Welcome to MCAT Basics [01:07] Topics covered in this episode [02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC) [28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations [54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics.